Tandem transcript translation

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Tandem transcript translation

F indings on page 747 suggest that multiple subunits of coiled-coil proteins may meet even before each subunit is fully translated. The article from Chang et al. reveals that the precursors to intermediate fi laments (IFs) assemble at tiny translation factories containing several mRNAs. Dimers of IFs, such as the neuronal protein peripherin, form into higher-order assemblies. Before they form f...

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Roles for transcript leaders in translation and mRNA decay revealed by transcript leader sequencing.

Transcript leaders (TLs) can have profound effects on mRNA translation and stability. To map TL boundaries genome-wide, we developed TL-sequencing (TL-seq), a technique combining enzymatic capture of m(7)G-capped mRNA 5' ends with high-throughput sequencing. TL-seq identified mRNA start sites for the majority of yeast genes and revealed many examples of intragenic TL heterogeneity. Surprisingly...

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Translation with frameshifting of ribosome along mRNA transcript

Translation is an important process for prokaryotic and eukaryotic cells to produce necessary proteins for cell growth. Numerious experiments have been performed to explore the translational properties. Diverse models have also been developed to determine the biochemical mechanism of translation. However, to simplify the majority of the existing models, the frameshifting of ribosome along the m...

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Tandem translation starts in the cheA locus of Escherichia coli.

The cheA locus of Escherichia coli encodes two protein products, CheAL and CheAS. The nucleotide sequences of the wild-type cheA locus and of two nonsense alleles confirmed that both proteins are translated in the same reading frame from different start points. These start sites were located on the coding sequence by direct determination of the amino-terminal sequences of the two CheA proteins....

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Alternative Translation Initiation of a Haloarchaeal Serine Protease Transcript Containing Two In-Frame Start Codons.

UNLABELLED Recent studies have shown that haloarchaea employ leaderless and Shine-Dalgarno (SD)-less mechanisms for translation initiation of leaderless transcripts with a 5' untranslated region (5' UTR) of <10 nucleotides (nt) and leadered transcripts with a 5' UTR of ≥10 nt, respectively. However, whether the two mechanisms can operate on the same naturally occurring haloarchaeal transcript c...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2006

ISSN: 1540-8140,0021-9525

DOI: 10.1083/jcb.1725iti4